Agent O was one of the many agents cramped in an elevator Agent Pinky attempted to use. Rather than use it, Agent Pinky instead decided to wait for another elevator ("Isabella and the Temple of Sap").
At one point in the desert, Agent O hid its head under the ground for unknown reasons ("Secrets from OWCA: The Organization Without a Cool Acronym").
- In "Where's Perry? (Part Two)", there were two ostrich agents meaning that the O.W.C.A. has more than one member of an animal species.
- This is confirmed in Agent O's secret file.
- In the secret file, it's also stated that Agent O works well when its head is not buried
- "Isabella and the Temple of Sap" (First appearance)
- "Secrets from OWCA: The Organization Without a Cool Acronym"
- "Where's Perry? (Part Two)" (other ostrich agents in Africa)
- "One Good Turn"
- "Phineas and Ferb Save Summer"
- "O.W.C.A. Files"
- Where's My Perry? (Agent File)